Fig. 3

Casp8 knockout induced less CD8⁺ T cell infiltration and poor response to anti-PD-1 treatment in B16F10 mice models. B16-C8KO or control cells (2 × 10⁵) were subcutaneously inoculated into the right flanks of C57BL/6 mice. Mice received 200 µg of intraperitoneal anti-PD-L1 monoclonal antibody or the equivalent isotype control antibody on days 4, 7, and 10. Tumor volume was monitored twice per week. a Experimental schematic of the B16F10 mouse model. b and c Tumor growth curves of the indicated groups (b) and each tumor (c). d The overall survival in the indicated groups. The mice were sacrificed when the tumor volume reached 2000 mm³. e and f CD4⁺ and CD8⁺ tumor-infiltrating cells in subcutaneous xenografts were analyzed by flow cytometry. e Representative flow cytometry of tumor-infiltrating T cells pre-gated for viable CD45⁺ cells. f Portions of T cells among CD45⁺ leukocytes in tumors