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Fig. 5 | Experimental Hematology & Oncology

Fig. 5

From: ALKBH5 prevents hepatocellular carcinoma progression by post-transcriptional inhibition of PAQR4 in an m6A dependent manner

Fig. 5

PAQR4 promoted the migration and invasion capability of HCC cells in vitro and promoted HCC metastasis in vivo. Transwell and wound healing assays showed that overexpression of PAQR4 promote the capability of migration and invasion in Hep3B and HLF cells (A, C and Additional file 2A and B). Knockdown of PAQR4 suppress the capability of migration and invasion in HLF and 97H cells (B, D and Additional file 2A and B). Immunofluorescence and Western blot analysis EMT markers like E-ca, N-ca, Vimentin in Hep3B and HLF cells (E, F). Representative images of intrahepatic metastasis model using PAQR4-overexpressing HLF cells (n = 5), HLF-Vector cells were used as control (G). The numbers of metastasis nodules (H). The Immunohistochemistry (IHC) assays showed expression of EMT markers E-ca and N-ca on tumor sections. Scale bar, 20 μm (40 ×). i *P < 0.05, **P < 0.01, ***P < 0.001

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Experimental Hematology & Oncology

ISSN: 2162-3619

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