Fig. 4

PAQR4 promoted HCC cells proliferation in vivo and in vitro. CCK8 and EdU assays confirmed that overexpression of PAQR4 promote proliferation in Hep3B and HLF cells (A, C and E), whereas knockdown of ALKBH5 repressed cell proliferation in HLF and 97H cells (B, D and E). F Representative images of tumor xenograft models using PAQR4-overexpressing HLF cells (n = 5), HLF-Vector cells were used as control. (G and H) Tumor weight and volume of PAQR4-overexpressing and control group were shown. I The Immunohistochemistry (IHC) assays showed expression of ki-67 and PCNA in the tumor xenograft model. Scale bar, 20 μm (40 ×). *P < 0.05, **P < 0.01, ***P < 0.001