Table 1 Primary and secondary antibodies used for Western blot
From: Alpha-B-Crystallin overexpression is sufficient to promote tumorigenesis and metastasis in mice
Antibody | Company | Cat. No | Dilution |
|---|---|---|---|
Alpha B Crystallin | Novus | NBP2-49,246 | 1:500 |
Basigin (CD147) | Abcam | ab230921 | 1:1000 |
GAPDH | R&D Systems | RDS2275PC100 | 1:2000 |
Vinculin | Cell signalling technology | 13,901 | 1:2000 |
β-Catenin | Cell signalling technology | 9582 | 1:1000 |
Cleaved Caspase-3 | Cell signalling technology | 9664 | 1:500 |
pAKTS473 | Cell signalling technology | 4060 | 1:1000 |
AKT | Cell signalling technology | 9271 | 1:1000 |
p-JNK | Cell signalling technology | 4671 | 1:1000 |
Rabbit secondary (peroxidase) | Sigma Aldrich | A0545 | 1:5000 |
Mouse secondary (peroxidase) | Sigma Aldrich | A9044 | 1:5000 |