Fig. 1
From: Alpha-B-Crystallin overexpression is sufficient to promote tumorigenesis and metastasis in mice
Cryab overexpression causes spontaneous tumorigenesis in vivo. a Schematic representation of αB-Crystallin overexpressing mice (Ozgene) developed by knocking in the transgene into the Rosa26 locus. The targeting vectors show recombination steps. Legend: FLPe recombination, Cre recombination, Homology arm, ▼ EcoRV site, transgene probe (tgP), 3’ probe (3’P), Neomycin probe (Neo). b Kaplan–Meier survival analysis of mice for indicated genotypes (n = 40 per group) Log-rank (Mantel–Cox) test was performed to determine P-value < 0.00001. c Percentage of cancer incidence rate among mice of indicated genotypes (n ≥ 40 per group); Fischer exact test was performed to determine P-value < 0.00001 (****). d The distribution of tumor spectrum in tissues of Cryab transgenic mice. e Representative images of gross morphology of tissues with different malignancies. (Scale bars, 1 cm)