Fig. 1

Low expression of pseudogene PEBP1P2 contributes to metastasis of ccRCC. a, b Real-time PCR analysis of PEBP1P2 RNA levels was conducted on 21 pairs of ccRCC tissues and corresponding non-cancerous tissues (a), and relative RNA level of PEBP1P2 was normalized to internal control 18 s rRNA (b). c, d The RNA level of PEBP1P2 was measured according to all samples (c) and the paired samples (d) in the ccRCC dataset TCGA database. e, f FISH detection of PEBP1P2 (green) RNA in ccRCC tissues and its adjacent non-cancerous tissues was performed, and nuclei were visualized with DAPI counterstaining (blue). The relative fluorescence intensity was shown as a scatter plot (e). g–i Kaplan–Meier curve was conducted to estimate overall survival (g), progress-free interval (h) and disease-specific survival (i), with a 95% confidence interval (dashed lines). j Receiver operating characteristic (ROC) analysis was constructed for quantifying response prediction. k Gross view of lung samples containing metastatic nodules (cyan arrows, metastatic nodules). l Slides of lungs stained with hematoxylin and eosin. m Number of the metastatic pulmonary lesions. The data are presented as the mean ± SD, **P < 0.01, ***P < 0.001