RNA binding proteins in MLL-rearranged leukemia

Tran, Tiffany M.; Rao, Dinesh S.

doi:10.1186/s40164-022-00343-5

Experimental Hematology & Oncology

Table 1 Characterized RBPs in MLL-AF9 and MLL-AF4 Leukemia

From: RNA binding proteins in MLL-rearranged leukemia

RBP	MLL FP	Type of leukemia	Mechanism	Function	Characterization	Refs.
METTL3	MLL-AF9	AML	RNA modification	m6A writer	Human primary AML cells, Human MOLM-13 cell line, MLL-AF9 Flt3-ITD mouse model, CRISPR dropout screen	[51, 52]
METTL14	MLL-AF9	AML	RNA modification	m6A writer	Human primary AML cells, Human MM6 cell line, MLL-AF9 Mettl14 CKO mouse model	[53]
FTO	MLL-AF9	AML	RNA modification	m6A eraser	Human primary AML cells, Human MONOMAC-6 and MV4-11 cell lines, MLL-AF9 Fto knockdown and heterzygous knockout mouse model	[54]
ALKBH5	MLL-AF9, MLL-AF4	AML	RNA modification	m6A eraser	Human primary AML cells, Human MONOMAC-6, NOMO1 MOLM-13, THP-1, MV4-11 cell lines, MLL-AF9 Alkbh5 CKO mouse model	[55, 56]
YTHDF2	MLL-AF9	AML	RNA modification	m6A reader	Human primary AML cells, Human THP-1 cell line, Ythdf2 CKO Hoxa9 Meis1 mouse model	[57]
METTL1	MLL-AF9	AML	RNA modification	m7G writer	Human primary AML cells, Human MOLM-13 and THP-1 cell lines, MLL-AF9/Flt3^ITD/+ deletion mouse model, MLL-AF9 AML xenograft mouse model	[63]
ADAR1	MLL-AF9	AML	RNA editing	A-to-I editing	Human primary AML cells, Human THP-1 cell line, MLL-AF9 Adar1 CKO mouse model	[72, 73]
RBM39	MLL-AF9	AML	Alternative splicing	Splicing factor	RBD CRISPR/Cas9 screen, Human primary AML cells, Human MOLM-13 and THP-1 cell lines, MLL-AF9 NrasG12D mouse model	[81]
DCPS	MLL-AF9, MLL-AF4	AML	Alternative splicing	5′ cap binding enzyme	Human MOLM-13, THP-1, and MV4-11 cell lines, Genome-wide CRISPR/Cas9 screen using MLL/AF9-Cas9 mouse model, PDX AML models	[84]
MBNL1	MLL-AF9, MLL-AF4	ALL, AML	Alternative splicing	Splicing, mRNA decay	Human primary MLL-r leukemia cells, Human THP-1, RS4;11, MOLM13, SEM and MV4-11 cell lines, MLL-Af4 mouse model, MLL-AF9 PDX with Mbnl1 knockdown mouse model, MLL-AF9 Mbnl1 KO mouse model	[86, 87]
DROSHA	MLL-AF9, MLL-AF4	AML, ALL	Primary miR processing	miR biogenesis	Human primary AML cells, Human SEMK2, PER377, MV4-11 cell line, Mll^PTD/wt/Flt3^ITD/ITD mouse model	[95, 96]
NCL	MLL-AF9, MLL-AF4	AML	mRNA stability and 3′UTR association	Ribosome biogenesis, miR biogenesis	Human primary AML and ALL cells, Human MOLM-13 and MV4-11 cell lines, Cell-line MLL-AF9 xenograft NCL knockdown mouse model	[108, 112, 113]
ZFP36L1	MLL-AF9	AML	mRNA stability and 3′UTR association	MZ B cell maintenance, thymopoiesis	Human primary MLL-r AML cells, Human THP-1 cell line	[114]
LIN28B	MLL-AF9	AML	Multiple mechanisms of action	Embryonic stem cell pluripotency, self-renewal, fetal lymphopoiesis, miR biogenesis	Human MLL-r AML cells, Human THP-1 cell line, AML xenograft mouse model, MLL-AF9 AML mouse model	[120, 121]
MSI2	MLL-AF9	AML	Multiple mechanisms of action	Self-renewal and pluripotency of embryonic stem cells, regulation of HSCs	Human primary AML cells, Human NOMO-1 and THP-1 cell lines, MLL-AF9 AML Msi2 conditional knockout mouse model	[135, 136, 137, 139]
SYNCRIP	MLL-AF9	AML	Multiple mechanisms of action	Regulation of transcripts involved in myeloid leukemia stem cells	Human primary AML cells, Human NOMO-1, MOLM-13, and THP-1 cell lines, MLL-AF9 leukemia in vivo shRNA screen, MLL-AF9 AML Syncrip KO mouse	[138]
IGF2BP3	MLL-AF4	ALL	Multiple mechanisms of action	Cell migration, survival, differentiation and stem cell renewal	Human primary ALL cells, Human RS4;11 and SEM cell lines, IGF2BP3 enforced expression mouse model, MLL-Af4 Igf2bp3 KO leukemia model	[143, 58]

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ISSN: 2162-3619

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