Fig. 7

TEPP-46 ameliorates C. tropicalis-aggravated CAC. A Schematic showing the experimental design, timeline of AOM/DSS-induced CAC model (n = 6 for each group). B The representative pictures of colon tumors, the inside of the colon and spleens were displayed. C Tumor number, size, and load in each mouse were detected. D The spleen weight of each mouse was measured. E Representative histological images of colon tumors by H&E staining. Histological score was evaluated by a pathologist. Scale bars, 0.1 mm. F The expression of Ki-67 and PCNA in colon tumors tissue was detected by immunohistochemistry (IHC). The percentages of Ki-67-positive and PCNA-positive tumor cells were quantified. Scale bars, 50 μm. G The infiltration of CD11b⁺ myeloid cells and the level of PKM2 (p-Y105) of CD11b⁺ myeloid cells in colon tumors tissue were determined by immunofluorescence assay. Scale bars, 50 μm. The results shown here are expressed as the mean ± SEM. Each panel is a representative experiment of 6 independent biological replicates. *p < 0.05, **p < 0.01, ***p < 0.001. The following statistical analyses were performed: unpaired Student’s t-test or one-way ANOVA where appropriate