Fig. 2

Dectin-3 mediates C. tropicalis-induced glycolysis activation in MDSCs. A Heatmaps showing the upregulation of metabolites of the glucose metabolism in MDSCs treated with C. tropicalis (MOI = 1) for 24 h (n = 5). B Metabolic pathway enrichment analysis shows the pathways with the significant change. C WT MDSCs were stimulated with heat-inactivated C. tropicalis (MOI = 1) for 24 h. The mRNA expression of glycolytic enzymes was measured by q-PCR. D WT and Clec4d^−/− MDSCs were stimulated with heat-inactivated C. tropicalis (MOI = 1) for 48 h. Cell lysates were analyzed by immunoblotting for glycolytic enzymes. E WT MDSCs were stimulated with heat-inactivated C. tropicalis (MOI = 5) for 24 and 48 h. Lactate secretion by MDSCs were determined by lactate assay kit. F WT and Clec4d^−/− MDSCs were stimulated with heat-inactivated C.tropicalis (MOI = 5) for 48 h. Lactate secretion and glucose consumption by MDSCs were determined by lactate and glucose assay kit. G WT and Clec4d^−/− MDSCs were stimulated with or without C.tropicalis (MOI = 2), then the ECAR level of these cells was measured by Agilent Seahorse XFe96 Analyzer. H WT and Clec4d^−/− MDSCs were stimulated with heat-inactivated C. tropicalis (MOI = 1) for 6 h. Hif-1α mRNA expression were measured by q-PCR. WT and Clec4d^−/− MDSCs were stimulated with heat-inactivated C. tropicalis (MOI = 2) for 42 h. Cell lysates were analyzed by immunoblotting for HIF-1α. The results shown here are expressed as the mean ± SEM. Each panel is a representative experiment of at least three independent biological replicates. *p < 0.05, **p < 0.01, ***p < 0.001. The following statistical analyses were performed: unpaired Student’s t-test or one-way ANOVA where appropriate