Fig. 2
In-vivo thioredoxin deletion affects the self-renewal and differentiation of hematopoietic stem cells in a murine model. A, B Colony forming units. BM cells (2X104) per dish were plated in Meth3434 semi-solid medium following harvesting at day 10 of TAM injection, and CFU were counted at day 12. Data represented mean ± s.d. C, D Various hematopoietic stem/progenitor cell subsets: TXN1fl/fl (control) and ROSA-CreER-TXN1fl/fl mice were treated with tamoxifen (75 mg/kg) i.p. daily for 5 days. Mice were sacrificed at day 10 and various hematopoietic stem cell populations (LSK, SLAMKSL, LT-HSC, ST-HSC, MPP, CMP, GMP, MEP and CLP) in the bone marrow were quantitated. Total number of cell populations per a femur and a tibia were shown. E–G the differentiated of erythroid and myeloid derived from HSCs were characterized by flow cytometry. Data represented mean ± s.d, n = 7–8, * P < 0.05, **P < 0.01, ***P < 0.001). H, I LSK cells in mice were labeled with BrdU in vivo, followed by FACS analysis to assess the cell cycle profile. Data are representative of three independently performed experiments