Fig. 1
In-vivo thioredoxin deletion is lethal and is associated with reduced splenocytes and bone marrow cells in a murine model. A Mice with floxed TXN1 were crossed with mice carrying the ROSA-CreER transgene to develop an inducible model for thioredoxin deletion. TXN1fl/fl (control) and ROSA-CreER-TXN1fl/fl mice were given TAM (75 mg/kg) i.p. daily for 5 days. Mice were sacrificed at day 10. Thioredoxin was measured by Western blot in bone marrow cells. B Kaplan–Meier survival curve. TXN1fl/fl mice and ROSA-Cre-TXN1fl/fl mice were treated with TAM (75 mg/kg) i.p. daily for 5 days. Animal survival was monitored daily. C The weight of the mice as described in B was measured, TXN1 deletion resulted in drastic weight loss (humane endpoints required euthanasia following a > 20% reduction in body weight). D Total BM cells (one femur and one tibia) and total thymic cells following harvesting at day 10 of TAM injection. E Number of spleenocytes following harvesting at day 10 of TAM treatment. F Spleen weight (g) at day 10 of TAM treatment (n = 10 mice for each group each experiment, one of 3 separate experiments was shown). G Histology of bone marrow. Bone was harvested at day 10 from the mice as described in F. H Histology of spleen: Spleen was harvested at day 10 from the mice as described in F