Fig. 6
The Wnt/β-catenin signaling pathway is involved in G6PD-induced Dexa resistance in MM. a Volcano plot showing the differentially expressed genes (DEGs) identified by RNA-seq between the WT and G6PD-OE MM cells. b KEGG pathway analysis indicated that the DEGs were primarily enriched in the Wnt/β-catenin signaling pathway, the top enriched KEGG pathway. c, d WB analysis showed that the expression of G6PD was positively correlated with β-catenin in G6PD-OE (c) or G6PD-KD (d) MM cells. e–f WB analysis confirmed that Wnt3a rescued the decrease in β-catenin expression in ARP1 (e) and H929 (f) G6PD-KD cells. g, h Supplementation of Wnt3a decreased the ROS levels in ARP1 (g) and H929 (h) cells treated with RRX-001. i WB analysis of β-catenin levels in G6PD-OE cells treated with H2O2 and Dexa individually or combined. *p < 0.05 was considered statistically significant