Fig. 2

Elevated G6PD expression promotes cellular proliferation in MM cells and in the xenograft mouse model. a WB analysis confirmed that G6PD-OE MM cells successfully expressed upregulated levels of G6PD. b MTT assays were used to measure cell viability in WT and G6PD-OE MM cells. c, d Cell cycle distribution in WT and G6PD-OE cells was determined using flow cytometry (c) and the results are presented as histograms (d). e–g Increased G6PD expression significantly increased tumor growth (e, f) and tumor weight (g) in the xenograft mouse model. The difference between groups was calculated using a Student’s t-test. *p < 0.05, **p < 0.01 and ***p < 0.001 were considered statistically significant