Fig. 5
MHY4571 inhibits tumor progression in a mouse xenograft and orthotic model. A After NCI-H1703 cell injection, mice harboring human lung tumors were treated with vehicle (control) or MHY (5, 10, and 20 mg kg−1). Tumor growth was measured in control-treated and MHY-treated groups (n = 6 control; n = 6 for each MHY concentration). Body weight and tumor mass were measured. B Immunohistochemical staining for p-PKA and p-CREB, as described in the Methods. C H&E staining and TUNEL assays were performed in control-treated and MHY-treated groups. Data are shown as the means ± SD. Statistical significance is indicated as **** P < 0.0001. D Representation of the bioluminescent signal at pre-treatment, 1 week, 2 weeks, and 3 weeks after MHY treatment (10 mg kg−1; n = 5 and 20 mg kg−1; n = 5). Statistical significance is indicated as ** P < 0.01