Fig. 3

MHY4571 induces apoptosis in human lung cancer cells (A). The normal broncho-epithelial cells (BEAS-2B) and four different human squamous lung cancer cells were treated with increasing concentrations of MHY4571 for 24 h, and then the percentage of cell viability was determined by the MTT assay. B NCI-H1703 cells were treated with MHY4571 for 8 h, and the medium was replaced with a fresh medium. After incubation for 10 days, colonies were stained with crystal violet. C NCI-H1703 cells were treated with variable concentrations of MHY4571 for 24 h. The cells were stained with Annexin V-FITC/PI and analyzed by flow cytometry. D NCI-H1703 cells and HCC95 were incubated with MHY4571 as indicated for 24 h. The blots were probed with antibodies against P-Akt, Akt, P-ERK, and ERK. β-actin was used as an internal control. E PARP and procaspase-3 were detected to determine the apoptotic effects of MHY4571. Data are the means ± SD (n = 3). Significance was calculated by the student's t-test (*P < 0.05, **P < 0.01, and ***P < 0.001 vs. vehicle-treated cells)