Fig. 1

The BMIF and the PBP are molecularly distinct. A Cellular composition of the BMIF and PBP compartments at Dx and D29. Stacked bars represent the proportion of leukemic and non-leukemic cell blasts in each B-ALL patient. B Schematic summarizing specimen collection and analyses. Bone marrow interstitial fluid (BMIF) and peripheral blood plasma (PBP) were collected at diagnosis (Dx) and after post-induction therapy (D29) for 8 pediatric B-ALL patients. Samples were processed for proteomic, terminomic, metabolomic, and lipidomic analyses. B-ALL #04 and B-ALL #05, did not have sufficient material for metabolomic and lipidomic analysis. C Sample preparation workflows for multi-omic mass spectrometric analysis. D–G Principal Component Analysis (PCA) of (D) proteomics (E) metabolomics (F) lipidomics, and (G) terminomics data. Individual samples are plotted as circles and the mean principal components of experimental group means are plotted as squares