Fig. 2

Lineage tracing by CellTagging and mitochondrial DNA mutations. A Correspondence between cells before and after treatment with PlaB was traced by CellTagging (up) and mitochondrial DNA mutations (down). Each horizontal line stands for a unique CellTag group (up) or a similar mitochondrial DNA mutation group (down). B-NK cells: B cells and NK cells. EBM cells: Eosinophils, Basophils and Mast cells. MD cells: monocytes and dendritic cells. B The visualization of major trajectories of progenitor cells and their derived cells was defined. Cells were traced by CellTagging (up) or mitochondrial DNA mutations (down). Charts were colored according to cell types or pseudo-time values. C Sankey diagram (left) showed induced MPPs’ origins. PCA diagram (right) showed the correlations among HSCs, CLPs, CMPs, GMPs, and MEPs before PlaB treatment and induced MPPs. D The visualization of clustering and PAGA trajectories of the cells derived from progenitor cells with PlaB treatment. They were traced by CellTagging (up) or mitochondrial DNA mutations (down). Charts were colored by cell types. The size of the circle represented the cell quantity and the thickness of the line represented the correlation between clusters. E Schematic model of the PlaB-induced human hematopoietic progenitor cell reprogramming toward stemness