Fig. 2

KW-2478 induced cell cycle arrest in the G2/M phase in CML cells. a After K562 and K562/G01 cells were treated with KW-2478 for 48 h, the cell cycle distribution was determined using PI staining and flow cytometry. b Following flow cytometry detection, the cell proportions in each cell cycle phase were summarized. c Levels of expression of cell cycle-related proteins, such as cyclin B1, p21 and p27, were quantitatively analysed by western blots. The results are expressed as the mean ± SD. *P < 0.05, **P < 0.01