Fig. 5

RIDD targets in MM. A Representative agarose gel electrophoresis of the PCR product surrounding the XBP1 splice site in H929. B BLOC1S1 mRNA measured by qRT-PCR. C mRNA levels of the indicated genes were determined by qRT-PCR. H929 cells were treated with different ER-stress-inducers in the presence or absence of 4μ8c. D Levels of IRE1 substrates in H929 and MM1S after 4 h and 16 h of thapsigargin treatment. The amount of each protein was quantified by densitometry, normalized to GAPDH and plotted against time to determine the half-lives of IRE1 substrate proteins (t1/2). E Protein levels of the indicated genes in H929 12 h after the treatment with thapsigargin in the presence or absence of the 4μ8c. All results are presented as the means ± SD of three experiments. (*p < 0.05, **p < 0.01, ***p < 0.001)