Fig. 8

MiR-449a targeted regulation of HOXA13 expression. a Relative miR-449a expression in the normal gastric epithelial cell line GES-1 and GC cell lines by qRT-PCR. b Relative HOXA13 expression in the normal gastric epithelial cell line GES-1 and GC cell lines by qRT-PCR. c The expression of miR-449a and HOXA13 in GC and adjacent normal tissues by qRT-PCR. d Pearson’s correlation between miR-449a and HOXA13 based on the qRT-PCR results. e The regulation of HOXA13 expression produced by miR-449a. f Predicted binding sites between the HOXA13 3′-UTR and miR-449a. g Relative luciferase activity of the predicted wild-type or mutant binding site of miR-449a in the 3′-UTR of HOXA13 mRNA. h The angiogenic ability of GC cells was detected by tube formation assay. Original magnification, ×200. i, j Transwell assays were conducted to assess GC cell migration and invasion ability. Original magnification, ×200. k The expression of FAK/Src axis-related proteins in GC cells after transfection with miR-449a mimics or inhibitor was detected by Western blotting. l The protein expression of EMT-related markers in GC cells after transfection with miR-449a mimics or inhibitor was detected by Western blotting (*p < 0.05, **p < 0.01, ***p < 0.001, ^#p > 0.05)