Fig. 6

FN1 promoted malignant phenotypes in GC cells. a CCK-8 assay was used for GC cell proliferation. b Flow cytometry analysis was used to evaluate GC cell apoptosis. c Angiogenesis ability of GC cells was detected by tube formation assay. Original magnification, ×200. d, e Transwell assays were conducted to assess GC cell migration and invasion ability. Original magnification, ×200. f Whole SGC-7901 and MKN45 cell lysates were immunoprecipitated with antibodies against FN1 or IgG, followed by immunoblotting with antibodies against FN1, ITGA5, ITGB1 and GAPDH. g The protein expression of Bcl2, Bax, cleaved forms of Caspase-9, Caspase‐3, PARP and EMT-related markers was detected by Western blot analysis. h Western blot analysis of the FAK/Src axis-related proteins (**p < 0.01, ***p < 0.001)