Fig. 2

Single-cell RNA-seq analysis revealed molecular characteristics of TAMs in distant tumor types. a The t-SNE plot displays infiltrated TAMs. Each dot represents a macrophage and colors represent different clusters of cells. Red, green, and blue one separately represents for cluster 0, 1, and 2. b Scatterplot illustrating the expression of TAM markers in each cell clusters. Average expression of each subcluster is represented by color gradient, in which lower expression is represented by lighter color and higher expression is represented by darker color, and the percent of cells is represented by the size of dots. c–e AUCell R package to identify TAM cluster responses to “Ferroptosis gene sets”. (c cluster 2; d cluster 1; e cluster 0). f Construction of TAM differentiation trajectories through pseudotime analysis. g, h Expression patterns of marker genes enriched in different TAMs clusters in various tumors (g melanoma; h head neck squamous cancer)