Fig. 2
From: Targeting phosphoinositide 3-kinases and histone deacetylases in multiple myeloma
CUDC-907 activity in the myeloma cells. a Time course of HDAC activity analyzed using the HDAC Activity Assay Kit. Fluorescence intensity signals were measured using an EnSpire Multimode Plate Reader (Ex/Em 355/460 nm). *P < 0.05 vs. control. b Histone acetylation status in CUDC-907-treated (for 24 h) myeloma cells. Cellular histone acetylation was analyzed using the CycLex® Cellular Histone Acetylation Assay Kit. *P < 0.05 vs. control. c Myeloma cells were treated with vorinostat, pictilisib, vorinostat plus pictilisib, and CUDC-907 for 72 h. Cell growth was evaluated using the Cell Counting Kit-8. *P < 0.05 vs. CUDC-907-treated group. d Cells were treated with the indicated concentrations of CUDC-907 for 24 h. Akt activity was analyzed using the AKT (Phospho) [pS473] Human ELISA Kit. *P < 0.05 vs. control. e U266 cells were treated with CUDC-907 for 24 h. Total cell lysates were evaluated by immunoblotting. Results represent the mean of three independent experiments.