Fig. 4

The effects of SEPHS1 knockdown or overexpression on SMAD2/3/4 expression in HCC cells. a HepG2 and SK-HEP-1 cells were transfected with siControl, siSEPHS1#1 or siSEPHS1#2, followed by western blot analysis of indicated proteins. b HepG2 and SK-HEP-1 cells were transfected with siControl or siSEPHS1, followed by quantitative RT-PCR analysis of indicated mRNA levels. The relative mRNA levels were plotted. Columns, mean ± SD (n = 3). *, p < 0.01. n.s., not significant. c HepG2 cells were transfected with siControl or siSEPHS1 and treated with or without cycloheximide (CHX), followed by western blot analysis of indicated proteins. d HepG2 cells were transfected with siControl or siSEPHS1 and treated with or without MG132, followed by western blot analysis of indicated proteins