Fig. 3
MOLM-13 cells exhibit synergistic phospho-inhibition upon co-incubation of PKC412 and daunorubicin. a Levels of apoptosis (AnnexinV+) in MOLM-13 cells 24 and 48 h of culture (n = 3). b Mass cytometry analysis of MOLM-13, quantified change in expression illustrated as log2 median intensity compared to control, n = 4. c Bar graph showing the FLT3/CD135 surface expression on MOLM-13 cells after 18 h of culture. Graph shown MFI fold expression compared to untreated control, n = 4. Treatment as indicated; PKC412 10 nM, Dauno 0.05 µM, PKC412 10 nM) plus Dauno 0.05 µM, AraC 5 µM, PKC412 10 nM plus AraC 5 µM. d ImageStream high resolution flow cytometry of FLT3/CD135. MOLM-13 cells were treated with PKC, daunorubicin (Dauno), cytarabine (AraC) or co-incubated as indicated in the figure. Fixable Viability Stain (FVS) were used to exclude dead cells. Mean ± SD, *, P ≤ 0.05 **, P ≤ 0.01, as measured by Student’s t test