Fig. 1

Mass cytometry defines synergistic phosphoprotein inhibition in co-cultures of midostaurin, daunorubicin and cytarabine. a Daunorubicin (Dauno) and cytarabine (AraC) in co-culture with midostaurin (PKC412; PKC). Average percentage of apoptotic MV4-11 cells (AnnexinV+) determined after 24 and 48 h of culture with midostaurin 10 and 100 nM (PKC412; pink and red line, respectively), daunorubicin 0.05 µM (Dauno; dark blue line) or co-culture with PKC412 (10 nM) plus Dauno (0.05 µM, PKC412 + Dauno; light blue dotted line), cytarabine 5 µM (AraC; dark green line) or PKC412 10 nM plus AraC 5 µM (PKC412 + AraC; light green dotted line). Untreated cells were used as control (black line). b viSNE was used to analyze MV4-11 cells cultured in vitro for 18 h with indicated inhibitors or untreated control. viSNE analysis was run on 6000 live intact single cells per sample. viSNE maps define cell populations based on intracellular markers, red indicate high expression and blue low expression. c Graph shows quantified change in expression illustrated as log₂ median intensity compared to untreated control. PKC412 10 and 100 nM (pink and red bar), Dauno 0.05 µM (dark blue bar) or co-culture with PKC412 10 nM plus Dauno 0.05 µM (PKC412 + Dauno; light blue bar), AraC 5 µM (dark green bar) or PKC412 10 nM plus AraC 5 µM (PKC412 + AraC; light green bar). Mean ± SD (n = 3). *, P ≤ 0.05 **, P ≤ 0.01, as measured by paired Student’s t test