Fig. 4

PHGDH inhibition reduces the intracellular redox capacity and gives a therapeutic advantage in vivo. a HMCLs were treated overnight with their corresponding NCT-503 IC50 or double the IC50 (IC50*2), and then GSH levels were measured. b GSH levels, c total NADP⁺ and NADPH, and d relative NADPH/NADP⁺ ratio in untreated INA6-KD cells was measured. Graph A represents two independent experiments while graphs B-D represent three independent experiments, all with minimum two replicates. e Schematic image of the experimental setup using the 5T33MM model. The mice were divided into four groups: vehicle (n = 9), NCT-503 (n = 9), bortezomib (n = 8), and combination (n = 6). f Spleens were weighed. g BMs were harvested for analysis of plasmacytosis on cytosmears. h M spike was analyzed by protein electrophoresis on collected serum. Error bars are ± SEM and *P ≤ 0.05, **P ≤ 0.01, ***P ≤ 0.001