Fig. 1

IL-6 derived from activated CART-19 cells triggers monocyte release of IL-6. a IL-6 mRNA levels in CART-19 cells measured by quantitative PCR. Regular CART-19 cocultured with Raji cells for 24 h, IL-6 mRNA levels were measured by quantitative PCR. Data are shown as the mean ± SD (n = 3). NS = no significant difference, *p < 0.05. b IL-6 cytokines level secreted into the supernatant. IL-6 protein levels in the supernatants of in CART-19 cells cocultured with Raji cells for 24 h, were measured using the Th1/Th2 Cytometric Bead Array Kit II. NS, no significant difference, ****p < 0.0001. c IL-6 protein levels in the supernatants of cultures of monocytes incubated with supernatants from CART-19/Raji cell cocultures, as measured using the Th1/Th2 Cytometric Bead Array Kit II. NC, untransduced T cells cocultured with Raji cells. d IL-6 protein levels in monocyte culture medium after the addition of recombinant IL-1, IL-2, IL-6 or IFN-γ. Data are shown as the mean ± SD (n = 3). Student’s t test was used to assess differences between groups. **p ≤ 0.01, ****p ≤ 0.0001