Fig. 5

G9a depletion inhibit proliferation of MM cells in vitro. a RNA-seq performed on KMS12BM transfected with lentiviruses expressing shNT or shG9a (n = 2). Gene set enrichment analysis plots of negative enrichment (FDR = 0.41) for NFκB pathway when comparing shG9a#1 and #2 versus shNT. NER = -1.0079; FDR q-value = 0.41. NES, normalized enrichment score. b Immunoblots show G9a, p-RelB, RelB and proapoptotic factor Bim (β-actin as loading control) in indicated MM cell lines infected with lentiviruses expressing shNT or shG9a. c Immunoblots show RelB expression levels in representative KMS12BM xenografts with transfected shNT or shG9a lentivirus. d mRNA expression of RelB and proapoptotic factors BMF, BCL2L11 (Bim) in JJN3 cells treated by DMSO control or UNC0642 at \(IC_{50}\) for 72 h. (n = 3; mean ± SD; Two-tailed Student’s t test compared with DMSO treated control; *P < 0.05 and ***p < 0.001). e Whole cell lysates from JJN3 cells treated by DMSO control or UNC0642 at \(IC_{50}\) for 72 h were analyzed for the indicated proteins by immunoblotting