Fig. 2

Sequence alignment of variant splice variant hCNT1-IR and normal hCNT1 transcripts. The top sequence is that of hCNT1-IR, while the bottom sequence corresponds to sequence of hCNT1. The initial and latter black bold face sequences are those of the initial and latter nucleotides of hCNT1-IR that are matched to corresponding to cDNA sequence of normal hCNT1 in black plain face nucleotides. In between these two regions and highlighted in yellow, are sequence of the retained intron 4 of hCNT1-IR in bold face purple, and the matching genomic sequence of normal hCNT1 in bold green sequence. Mutations in hCNT-IR sequence are indicated by red letters; insertions are indicated by blue letters, while deletions are indicated by cyan spaces. The skipped sequences are indicated by a blue dotted line