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Table 1 Sensitivity and specificity of Ipsogen JAK2 Mutant Kit (Qiagen, Germany) confirmed by sequencing, HRM, PCR–RFLP and, ARMS PCR methods

From: Development of a high resolution melting analysis assay for rapid identification of JAK2 V617F missense mutation and its validation

Sample Mutant Wild type Sensitivity (%) Specificity (%)
No. tests Positive No. tests Negative
Ipsogen JAK2 mutant Kit (Qiagen, Germany) confirmed by sequencing 25 25 20 20 100 100
ARMS 25 25 20 20 100 100
PCR–RFLP 25 25 20 20 100 100
HRM 25 25 20 20 100 100