Table 1 Sensitivity and specificity of Ipsogen JAK2 Mutant Kit (Qiagen, Germany) confirmed by sequencing, HRM, PCR–RFLP and, ARMS PCR methods
Sample | Mutant | Wild type | Sensitivity (%) | Specificity (%) | ||
|---|---|---|---|---|---|---|
No. tests | Positive | No. tests | Negative | |||
Ipsogen JAK2 mutant Kit (Qiagen, Germany) confirmed by sequencing | 25 | 25 | 20 | 20 | 100 | 100 |
ARMS | 25 | 25 | 20 | 20 | 100 | 100 |
PCR–RFLP | 25 | 25 | 20 | 20 | 100 | 100 |
HRM | 25 | 25 | 20 | 20 | 100 | 100 |