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Table 1 Culture systems of multiple organoids

From: Organoid technology in disease modelling, drug development, personalized treatment and regeneration medicine

Organoid type Sources Culture components Ref.
Lung Lung alveolar epithelial cells (together with lung fibroblasts) Matrigel
SAGM medium with all additives except epinephrine (Y27632 was added during the first 2 days. Wnt3A, FGF7, FGF10, XAV939 and CHIR99021 were added after the first 2 days)
Gastric hPSCs Matrigel
Advanced Dulbecco’s modified Eagle’s medium (DMEM)/F12 with N2, B27, l-glutamine, HEPES, penicillin/streptomycin and EGF (Retinoic acid and Noggin were added for the first 3 days)
Lgr5+ stem cells Matrigel
Advanced DMEM/F12 with N2, B27, N-acetylcysteine, Gastrin, and EGF, R-spondin-1, Noggin, FGF10 and Wnt3A (Y-27632 was added for the first 2 days, and Exendin 4 was added for the differentiation of enteroendocrine lineage)
Intestinal ESCs and iPSCs Matrigel
Advanced DMEM/F12, N2, B27, l-glutamine, HEPES, penicillin–streptomycin, EGF and Noggin
Liver iPSCs Matrigel
Hepatocyte culture medium supplemented with dexamethasone, oncostatin M, hepatocyte growth factor and SingleQuots
Pancreas Pancreatic progenitor from hPSCs Matrigel
MCDB131, glucose, sodium bicarbonate, FAF- bovine serum albumin, l-glutamine, l-ascorbic acid, FGF2, Y27632, insulin–transferrin–selenium–ethanolamine, nicotinamide, FGF10, glucose, indolactam V and the transforming growth factor β-receptor inhibitor SB431542
Prostate Luminal and basal prostatic epithelial lineages Matrigel
Advanced DMEM/F12, penicillin/streptomycin, GlutaMAX and Hepes, B27, N-acetylcysteine, EGF, the transforming growth factor-beta inhibitor A-83-01, Noggin, R-spondin-1, dihydrotestosterone, nicotinamide, FGF2, FGF10, SB202190 and prostaglandin E2 (Y-27632 is added when passaging with TrypLE)
Brain ESCs Matrigel
Advanced DMEM/F12, FGF2, insulin, Shh signaling inhibitor, bone morphogenetic protein, Y-27632, SB431542, FGF19 and stromal cell-derived factor 1