Fig. 3

PRL-3 knockdown reduced survival and migration of cHL cells. a Western blot of PRL-3 knockdown by shRNA in L1236 and HDLM2. b Knockdown of PRL-3 reduced viability in L1236, but not in HDLM2, measured by annexin V-FITC/propidium iodide using flow cytometry. Bars represent percent viable cells from one one of three independent experiments with similar results. Error bars represent + 1 SD of duplicates. *p < 0.05. ns not significant. c PRL-3 knockdown reduced cell proliferation in L1236, but not in HDLM2 measured by cell counting. Error bars represent ± 1 SD of duplicates. Figure is showing one of three independent experiments with similar results. d Western blot of Mcl-1 and Bcl-xL expression. Membrane was reprobed for Bcl-xL and GAPDH. e, f Knockdown of PRL-3 reduced migration in e L1236 and f HDLM2. Bars represent the percentage of migrated cells from one of three independent experiments with similar results. Error bars represent + 1 SD of three repeated counts in two parallels. ***p < 0.001