Fig. 3

Gata2 and p53 are down regulated in the LN. GATA2 and p53 gene expression was analysed by qRT-PCR in HSC from the different niches and compared to freshly isolated cells. RPS18 gene expression was used to normalize data and samples were run in triplicates (n = 3). a Gata2 gene expression. b p53 gene expression. Data are shown as mean ± SEM. p values were calculated by paired Student’s t test, (ns: non-significant, *p < 0.05, **p < 0.01)