Fig. 2

The LN increases proliferation of HSC. HSC proliferation, from the different niches as indicated, was evaluated by labelling cells with anti-Ki67 monoclonal antibody conjugated to FITC by flow cytometry (FACSAriaTM II, BD Bioscience). a Representative histogram showing fluorescence intensity of Ki-67. b Mean fluorescence intensity (MFI) quantification of HSC in NN10, NN1 and LN stained with anti-Ki-67 antibody. c qRT-PCR for the evaluation of c-Myc mRNA expression, as described in “Methods”. FlowJo was used for data analysis. Results shown are from two independent experiments done in duplicates. Error bars represent SEM *p < 0.05;**p < 0.01 (Student’s t test)