Fig. 2
From: Activation of NQO1 in NQO1*2 polymorphic human leukemic HL-60 cells by diet-derived sulforaphane
Effect of SFN on cell proliferation, viability and cell cycle control in HL-60 cells. a Cells were treated with 0, 10 and 25 μM SFN and the cell numbers were determined at 6, 15, 24 and 48 h by counting using a hemocytometer. Cell viability was measured using the trypan blue exclusion assay. Values are expressed as mean ± SD for three experiments, and asterisks * and ** shown above bars indicate statistical significance of p < 0.01 and p < 0.001, respectively, compared to vehicle-treated control. b, c Cells were treated with 0, 10 and 25 μM SFN for 24 and 48 h and analyzed by flow cytometry. The cells in sub-G1 phase are used to estimate apoptotic cells