Fig. 5
Thymocyte GSH in mice treated with sirolimus (Day 4). One representative from three triplicate experiments is shown. Mice received intraperitoneal DMSO or sirolimus on Days 0–3. On Day 4, thymus fragments were collected and processed immediately for measuring cellular GSH. The GS-bimane derivatives (retention time, R t = 7.7 min) were separated on HPLC and analyzed by fluorescence. The values of GSH (nmol mg−1) are shown