Figure 4

Comparison of histopathological changes in host tissues of the 3 groups. Lethally irradiated BALB/c host mice were given intravenous injections of 2 × 10^6 TCDBM cells from C57BL/6 donors with or without 0.25 × 10 ^6 CD4+ T cells from donors and 1 × 10 ^6 hMSCs. Multiple doses of hMSCs were given at the day of transplantation then 3d and 6d after transplantation. Host spleen, liver, colon and lung are harvested 14d after transplantation and hematoxylin and eosin sections were prepared for light microscopy. Diminished graft versus host response was noted upon treatment with hMSCs namely substantial white pulp cellular infiltration in the spleen, periportal inflammation in the liver, loss of crypts in the colon and peribronchial inflammation and alveolar congestion in the lungs. Each specimen is a representative of 3 mice from each treatment group. Magnification × 100 for panels A and D; × 200 for panels B and C.