Figure 6
Induction of DNA fragmentation in HL-60 cells. Lane 1: M-Molecular weight marker. Lane 2-Positive control marker/Camptothecin (5 μM). Lane 3- ATO alone (6 μg/mL). Lane 4- Control with no treatment. Lane 5- VitD3 (0.5 μM) + ATO (6 μg/mL ATO); Lane 6- VitD3 (1.0 μM) + ATO (6 μg/mL ATO); Lane 7- VitD3 (1.5 μM) + ATO (6 μg/mL ATO). HL-60 celles were treated for 24 hr. The DNA gel was stained with SYBR Green 1 after electrophoresis on a 1% agarose gel and then analyzed on the Typhoon 9400 phospho imager.