Figure 3

Combination of phenethyl isothiocyanate (PEITC) and paclitaxel (taxol) inhibits cell cycle regulator cyclin B1 and Cdk1 in MCF7 and MDA-MB-231 breast cancer cells. The MCF7 cells were treated with 5 μM of PEI and 10 nM of taxol alone or in combination. Due to higher resistance of MDA-MB-231 cells, PEI at 10 μM and taxol at 100 nM were used. Cyclin B1 and Cdk1 were immunoprecipitated with appropriate antibodies and were detected by Western blotting as described in Materials and methods. β-actin level was used as an internal loading control for protein amount. A representative blot from three or more independent experiments was shown.