Figure 2

Acetyl-alpha-tubulin immunofluorescence in the cytoplasm of MCF7 and MDA-MB-231 breast cancer cells. The MCF7 cells and MDA-MB-231 cells were treated with or without PEITC. The cells were stained with anti-acetyl-alpha-tubulin. Fluorescin-labeled secondary antibody was then added. The cells were visualized under confocal fluorescent microscope. The nuclei were counter stained with propidium iodide (PI). In the treated cells, acetylated alpha-tubulin was seen in the cytoplasm.