Effect of metronomic and standard chemotherapy on breast cancer patients. (A) Differential distribution of Treg/Teff ratio in the peripheral blood of breast cancer patients prior chemotherapy administration (Pre-CTx) and after metronomic or standard treatment. Shown are mean values ± SE. All samples were analyzed in duplicates. (B) Teff proliferation expressed as mean OD at 450 nm ± SE. (C) Treg-induced suppression on Teff proliferation. Shown are mean % values ± SE. All samples were analyzed in triplicates. Other details as in Figure 2. * p < 0.05 and ** p < 0.01 as estimated by Mann–Whitney or paired-t tests.