Figure 2
From: Silence of MCL-1 upstream signaling by shRNA abrogates multiple myeloma growth
Effect of shSTAT3, shPI3K and shMAPK1/2 on MCL-1 gene expression. Total RNA was isolated from human U266 and 8226 cells transfected with lentivirus carrying shSTAT3, PI3K shMAPK1, shMAPK2 or control shRNA in presence of IL-6 (48 hours). (A) mRNA expression of STAT3, PI3K and MAPK in U266 cells were quantified by qRT-PCR. The mRNA levels were presented as fold change in comparison with the control shRNA treatment group. (B) Protein expression of STAT3, PI3K or MAPK in U266 cells was determined by Western blot with specific antibodies against the protein. (C) mRNA levels of STAT3, PI3K and MAPK in 8226 cells were also quantified by qRT-PCR and presented as fold changes compared with the shRNA treatment control group. (D) MCL-1 mRNA expression in 8226 cells was quantified by qRT-PCR. The fold change of MCL-1 mRNA in targeted cells was compared with the control shRNA treatment group. *, P < 0.05; **, P < 0.01.