Figure 1

Assessment of the KIT^D816V mutation on the bone marrow trephine. A upper line: HE stain of the bone marrow trephine showing both, CMML and SM part in distinct areas in this patient (left). A CD117 immunostain (right) highlights the mast cells in the SM compartment, however cells within the CMML compartment are negative. Lower line: HE control before and after punching out the SM area (containing around 90% of MC in the CD117 staining) submitted to PCR and Sanger Sequencing. B Forward and reverse sequences indicating the KIT^D816V mutation of microdissected SM area of the bone marrow trephine. C FACS sorted cells as indicated with a purity of around 90% for CD34+ stem cells and granulocytes. D Shows the sequencing results of the PCR amplification product for KIT^D816V, and forward strands for isolated cell-subpopulations respectively.