Figure 4

Western blot of cell lysates from CLL patients and healhty control donors. The membrane/organelle fraction from non-progressive (n = 4) and progressive (n = 4) CLL patients and from one healthy control. Upper panel: a rabbit anti- C-terminal OPTC pAb detecting a 37 kDa band in the membrane/organelle fraction of CLL cells but not in healthy donor PBMC. Lower panel: The same membrane stripped and re-probed with an anti-β-actin mAb. Multiple bands were detected in the healthy control while in CLL patients a single 42 kDa band was noted.