Dose-dependent analysis of the MIA PaCa-2 cells to oxythiamine. A. Two-DE patterns of whole-cell proteins obtained from MIA PaCa-2-2 cells treated with oxythiamine (0 μM, 5 μM, 50 μM, and 500 μM). On the basis of PDQuest software analysis, ratios of normalized spot intensities of four different concentrations of oxythiamine were calculated, and spots showing more than 2.0-fold difference with statistical significance (p < 0.05) were selected after these 2D gels were stained by SYPRO Ruby. B. The validation of two differential proteins (alpha-enolase and 14-3-3 protein beta/alpha) for dose–dependent group. Western blot scores are annotated under the bands. The results were well consistent with the 2-DE analyses.