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Fig. 3 | Experimental Hematology & Oncology

Fig. 3

From: Targeting NRF2 uncovered an intrinsic susceptibility of acute myeloid leukemia cells to ferroptosis

Fig. 3

ML385 cooperates with FIN56/RSL3 to reduce the viability of AML cells. (a) MV4;11 cells were treated with the indicated concentrations of RSL3 (left panel) or FIN56 (right panel) for 24 h, and the expression of GPX4 was detected by western blotting. (b) AML cell lines and PBMCs from HIs were treated with the indicated concentrations of RSL3 for 24 h, and the cell viability was detected by the CCK8 assay. (c) MV4;11 (left panel) and Kasumi-1 cells (right panel) were treated with ML385 alone, RSL3 alone, or the combination of ML385 and RSL3 at the indicated concentrations for 24 h, and the cell viability was measured by CCK8 assay. CI values were calculated by the Chou-Talalay method. The dashed line designates a CI value of 1, and CI < 1 indicates a synergistic interaction between the two agents in the combination. (d) MV4;11 (left panel) and Kasumi-1 cells (right panel) were treated with ML385 alone, FIN56 alone, or the combination of ML385 and FIN56 at the indicated concentrations for 24 h, and the cell viability was measured by CCK8 assay. CI values were calculated by the Chou-Talalay method. The dashed line designates a CI value of 1, and CI < 1 indicates a synergistic interaction between the two agents in the combination. Data are expressed as the mean ± SD. n = 3 or more independent biological replicates, presented as individual points. P value < 0.05 was considered significant (a, one-way ANOVA with Bonferroni post hoc test; b, two-tailed unpaired Student’s t test).

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