Fig. 1
From: Targeting NRF2 uncovered an intrinsic susceptibility of acute myeloid leukemia cells to ferroptosis
Antioxidant and ferroptosis genes are validated as direct NRF2 targets. (a) The flow chart for screening potential NRF2 target genes is depicted, indicating the various steps involved in identifying and validating NRF2 target genes. (b) A Venn diagram analysis of four NRF2 Chip-seq cohorts is shown, indicating the overlap and unique genes identified by each cohort. (c) The top 10 enriched terms of the Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analyses of Chip-seq intersection genes are displayed, highlighting the most significant biological processes and pathways associated with NRF2 target genes. (d) A Venn diagram shows the overlapping target genes between NRF2 Chip-seq target genes and RNA-seq up-regulated genes, indicating potential candidate genes for further analysis. (e) A scatter plot of the top 20 enriched KEGG pathways of LV-NRF2 vs. LV-NC is shown in the bubble plot, highlighting the pathways significantly enriched in NRF2-overexpressing cells. (f) The relative expression of genes related to ferroptosis in NRF2-overexpressing MV4;11 cells were detected by qRT-PCR. (g) The expression of genes related to ferroptosis in NRF2-knockdown MV4;11 cells were detected by qRT-PCR. Data are expressed as the mean ± SD. n = 3 or more independent biological replicates, presented as individual points. P value < 0.05 was considered significant (f-g, two-tailed unpaired Student’s t test).