Fig. 8

Heterogeneity analysis of fibroblasts in primary and lymphatic metastatic PCa TME. (A). Subcluster of fibroblast distribution between metastatic and primary lesions using UMAP-2 analysis; (B). Mean percentage of fibroblast subclusters in primary and metastatic lesions; (C). Violin plots showing normalized expression levels of marker genes across fibroblast subclusters; (D). Tissue immunofluorescence showing CXCR4 + Fibroblasts in normal tissue, primary lesions and lymphatic metastases; red fluorescence representing SMA and green fluorescence representing CXCR4.(E).DEGs in metastasis were identified using edgeR package with comparison to primary lesion. Scatter plots showing respective DEG profiles in PCa. Red spots indicate up-regulated genes; green spots indicate no significant change in genes; (F). Functional enrichment analysis of fibroblast DEGs; (G). GSEA heatmap of 50 hallmark gene sets in MSigDB database among fibroblast subclusters