Fig. 7
LINP1 suppresses PERK/eIF2α axis-modulated UPR signaling and the following apoptosis.. A Detection of key protein expression in UPR signaling including p-eIF2α, GRP78, XBP1, ATF4, DDIT3 and DR5 in response to LINP1 knockdown under TM (2 µg/mL) treatment by Western blot. B Detection of key gene expression in UPR signaling including GRP78, XBP1, DDIT3 and DR5 in response to LINP1 knockdown under TM (2 µg/mL) treatment by qPCR. C Detection of ThT fluorescence intensity corresponding to ER stress-induced activation of the unfolded protein response after TM treatment and LINP1 knockdown. Scale bars, 50 μm. D, E After LINP1 depletion, cSCC cells was pretreated with PERK inhibitor GSK2656157 for 1 h and then treated with 2 µg/mL TM for 12 h. The expression level of the UPR signaling genes were determined by Western blot and qPCR. F Apoptosis rate was detected by Annexin V-APC/7-AAD double staining after LINP1 knockdown. Data are plotted as the means ± s.d. n = 3. *P < 0.05, **P < 0.01, ***P < 0.001. G Detection of key protein expression in UPR signaling including p-eIF2α, GRP78, XBP1, ATF4, DDIT3 and DR5 in response to LINP1 knockdown in HaCaT cells under the treatments of TM, ultraviolet B and H2O2